RETURNYonago Acta medica 1991;34:163-174
Identification and Pattern Analysis of Bile Acids in Swine Gallbladder Bile by Capillary Column Gas Chromatography and Gas Chromatography-Mass Spectrometry
Nobuo Yamaga, Shin-ichi Yoshioka, Tetsuo Mura, Tsutomu Koumi, Koji Kimura*, Toshiyuki Shibahara and Shiro Ikawa
Division of Chemistry, Institute of Steroid Research, *Department of Biochemistry and Laboratory Animal Research Center, Faculty of Medicine, Tottori University, Yonago 683, Japan
The identification and pattern analysis of bile acids in swine gallbladder bile were carried out by capillary gas chromatography and capillary gas chromatography-mass spectrometry. Thirteen peaks which seem to be bile acids were detected on a gas chromatogram simultaneously, and 9 of these peaks were identified with the relative retention time and mass spectrum as lithocholic acid, chenodeoxycholic acid, hyodeoxycholic acid, hyocholic acid, 3α-hydroxy-6-oxo-5α-cholan-24-oic acid, 3α-hydroxy-6-oxo-5β-cholan-24-oic acid, 3α,6β-dihydroxy-5β-cholan-24-oic acid, β-hyodeoxycholic acid and 3-oxo-7α-hydroxy-5β-cholan-24-oic acid. Predominant bile acids in swine gallbladder bile were chenodeoxycholic acid, hyodeoxycholic acid and hyocholic acid, and these bile acids comprised 81.20% of total bile acids in swine gallbladder bile.
Key words: bile acid; gas chromatographic pattern analysis; swine gallbladder bile