<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd"> <HTML> <HEAD> <META name="generator" content="Adobe InDesign HTML Export Plug-in"> <META http-equiv="Content-Type" content="text/html; charset=iso-8859-1"> <META http-equiv="Content-Style-Type" content="text/css"> <TITLE>yam49_039-047.html</TITLE> <STYLE type="text/css"> <!-- #layer1 {position: absolute; top: 9px; left: 18px; padding: 0px; margin: 0px; z-index: 1} .dynamic-style-1 {text-align: left} .dynamic-style-2 {font-family: 'Times'; font-size: 14px; line-height: 14px; color: #000000; font-weight: bold} .dynamic-style-4 {margin-top: 11px; margin-bottom: 8px; text-align: left} .dynamic-style-5 {font-family: 'Times'; font-size: 17px; line-height: 18px; color: #000080; font-weight: bold} .dynamic-style-6 {margin-bottom: 8px; text-align: left} .dynamic-style-7 {font-family: 'Times'; font-size: 16px; line-height: 18px; color: #000000; font-weight: bold} .dynamic-style-8 {margin-bottom: 14px; text-align: left} .dynamic-style-9 {font-family: 'Times'; font-size: 15px; line-height: 16px; color: #000000; font-style: italic} .dynamic-style-10 {font-family: 'Times'; font-size: 15px; line-height: 17px; color: #000000} .dynamic-style-11 {margin-bottom: 17px; text-align: left} .dynamic-style-12 {font-family: 'Times'; font-size: 14px; line-height: 14px; color: #000000; font-weight: bold} --> </STYLE> </HEAD> <BODY bgcolor="#F0FFFF"> <DIV id="layer1"> <DIV class="dynamic-style-1"> <SPAN class="dynamic-style-2">Yonago Acta medica 2006;49:39-47</SPAN> </DIV> <DIV class="dynamic-style-4"> <SPAN class="dynamic-style-5">Suppression of LPS-induced Cyclooxygenase-2 (<I>cox-2</I>) Gene Expression in Mouse Macrophages by Excretory/Secretory Products of <I>Spirometra erinaceieuropaei</I> Plerocercoids <BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-7">Soji Fukumoto, Paramasari Dirgahayu*, Kozue Nunomura, Haruyo Matsuura and Kazumitsu Hirai <BR></SPAN> </DIV> <DIV class="dynamic-style-8"> <SPAN class="dynamic-style-9">Division of Molecular Medical Zoology, Department of Microbiology and Pathology, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503 Japan; *Present address: Department of Parasitology, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia<BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-10">The escape mechanism of parasites from inflammatory processes has been thought to be one of the most important tools for their survival in infected tissues. On the other hand, inducible cyclooxygenase-2 (cox-2) has been shown to play a major role in the process of inflammation. We investigated the effect of excretory/secretory (ES) products from the plerocercoids of <I>Spirometra erinaceieuropaei</I> on <I>cox-2</I> gene expression in a murine macrophage cell line (RAW 264.7). Preincubation of the macrophages with the ES products inhibited LPS-induced <I>cox-2</I> mRNA expression by 75% as well as its protein production. Dibutyryl cAMP enhances LPS-induced <I>cox-2</I> mRNA expression. The ES products also inhibited this enhanced expression by 46%. Inhibition of p38 mitogen-activated protein kinase (MAPK) with SB203580 or that of extracellular signal-regulated protein kinase with PD98059 reduced LPS-induced <I>cox-2</I> mRNA expression by 75% or 52%, respectively, along with its protein production. This evidence suggests that both MAPK pathways are crucial for full induction of <I>cox-2</I> gene expression. One experiment using a transcriptional inhibitor, actinomycin-D, showed that the ES products destabilized LPS-induced <I>cox-2</I> mRNA in RAW 264.7 macrophages. These results show that ES products from the plerocercoids of <I>Spirometra erinaceieuropaei</I> suppress LPS-induced <I>cox-2</I> mRNA expression in murine RAW 264.7 macrophages, and may attenuate inflammation around the plerocercoids of <I>S. erinaceieuropaei</I>. <BR></SPAN> </DIV> <DIV class="dynamic-style-11"> <SPAN class="dynamic-style-12">Key words: <I>Spirometra erinaceieuropaei</I>; lipopolysaccharide; macrophage; cyclooxygenase-2; mitogen-activated protein kinase<BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-10"> <IMG SRC="../pdficonsmall.gif" ALIGN="MIDDLE" BORDER="0" HSPACE="10" X-SAS-IseImageWidth X-SAS-UseImageHeight> <A HREF="49_039-043.pdf ">Pages 39 to 43 with/without table(s) and graphic(s) in PDF (160 k)</A> <BR> <BR> <IMG SRC="../pdficonsmall.gif" ALIGN="MIDDLE" BORDER="0" HSPACE="10" X-SAS-IseImageWidth X-SAS-UseImageHeight> <A HREF="49_044-047.pdf ">Pages 44 to 47 in PDF (180 k)</A> <BR> <BR> <IMG SRC="../../backIcon.gif" ALIGN="middle" hspace="10"> <A HREF="49-1contents.html"> RETURN</A><BR><BR></SPAN> </DIV> </DIV> </BODY> </HTML>