<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd"> <HTML> <HEAD> <META name="generator" content="Adobe InDesign HTML Export Plug-in"> <META http-equiv="Content-Type" content="text/html; charset=iso-8859-1"> <META http-equiv="Content-Style-Type" content="text/css"> <TITLE>yam49_029-037.html</TITLE> <STYLE type="text/css"> <!-- #layer1 {position: absolute; top: 9px; left: 18px; padding: 0px; margin: 0px; z-index: 1} .dynamic-style-1 {text-align: left} .dynamic-style-2 {font-family: 'Times'; font-size: 14px; line-height: 14px; color: #000000; font-weight: bold} .dynamic-style-4 {margin-top: 11px; margin-bottom: 8px; text-align: left} .dynamic-style-5 {font-family: 'Times'; font-size: 17px; line-height: 18px; color: #000080; font-weight: bold} .dynamic-style-6 {margin-bottom: 8px; text-align: left} .dynamic-style-7 {font-family: 'Times'; font-size: 16px; line-height: 18px; color: #000000; font-weight: bold} .dynamic-style-8 {margin-bottom: 14px; text-align: left} .dynamic-style-9 {font-family: 'Times'; font-size: 15px; line-height: 16px; color: #000000; font-style: italic} .dynamic-style-10 {font-family: 'Times'; font-size: 15px; line-height: 17px; color: #000000} .dynamic-style-11 {margin-bottom: 17px; text-align: left} .dynamic-style-12 {font-family: 'Times'; font-size: 14px; line-height: 14px; color: #000000; font-weight: bold} --> </STYLE> </HEAD> <BODY bgcolor="#F0FFFF"> <DIV id="layer1"> <DIV class="dynamic-style-1"> <SPAN class="dynamic-style-2">Yonago Acta medica 2006;49:29-37</SPAN> </DIV> <DIV class="dynamic-style-4"> <SPAN class="dynamic-style-5">Effects of Endothelin-1 and Angiotensin-II on Extracellular Matrix Metabolism in a Rat Mesangial Cell Line, CRL-2573 <BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-7">Satoru Yamamoto, Masahiko Koda, Masaru Ueki, Chishio Munemura, Satoko Maeta and Yoshikazu Murawaki<BR></SPAN> </DIV> <DIV class="dynamic-style-8"> <SPAN class="dynamic-style-9">Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8504 Japan<BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-10">The profibrogenic role of endothelin-1 (ET-1) and angiotensin-II (AT-II) in renal fibrosis remains disputed. We therefore studied the effect of ET-1 and AT-II on rat mesangial cells (CRL-2573), the major source of extracellular matrix proteins in the kidney. ET-1 stimulated DNA synthesis assessed by BrdU uptake in microtiter tetrazolium assay in a dose dependent manner, but AT-II did not. Proliferation of the mesangial cells by ET-1 was suppressed by 15% with BQ123, an endothelin receptor A antagonist, which suggested a significant role of the endothelin receptor A on growth stimulation. In the mesangial cells, ET-1 increased the mRNA expressions of procollagen &alpha;1 (I), transforming growth factor-&beta;<sub>1</sub>, connective tissue growth factor, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-13 in dose dependent manners, whereas AT-II had exerted no effect on the expression of these mRNAs. These data suggested that ET-1 might be involved in renal fibrosis through the mesangial cell proliferation and/or the increase in fibrogenic cytokines. <BR></SPAN> </DIV> <DIV class="dynamic-style-11"> <SPAN class="dynamic-style-12">Key words: angiotensin-II; endothelin-1; extracellular matrix metabolism; mesangial cell<BR></SPAN> </DIV> <DIV class="dynamic-style-6"> <SPAN class="dynamic-style-10"> <IMG SRC="../pdficonsmall.gif" ALIGN="MIDDLE" BORDER="0" HSPACE="10" X-SAS-IseImageWidth X-SAS-UseImageHeight> <A HREF="49_029-033.pdf "> Pages 29 to 33 with/without table(s) and graphic(s) in PDF (192 k)</A> <BR> <BR> <IMG SRC="../pdficonsmall.gif" ALIGN="MIDDLE" BORDER="0" HSPACE="10" X-SAS-IseImageWidth X-SAS-UseImageHeight> <A HREF="49_034-037.pdf ">Pages 34 to 37 in PDF (172 k)</A> <BR> <BR> <IMG SRC="../../backIcon.gif" ALIGN="middle" hspace="10"> <A HREF="49-1contents.html"> RETURN</A><BR><BR></SPAN> </DIV> </DIV> </BODY> </HTML>