Yonago Acta medica 1997;40:73–77
An Examination of Alkaline Hydrolyzing Conditions of Conjugated Bile Acids with Carbonyl Groups
Nobuo Yamaga, Yumiko Nagano and Kazuo Yamada
Department of Biochemistry, Faculty of Medicine, Tottori University, Yonago 683, Japan
The conditions for alkaline hydrolysis of conjugated bile acids with carbonyl groups were investigated using several oxo bile acids and conjugated bile acids. Under the condition using a 1 N NaOH solution for 4 h in an autoclave, glycine- and taurine-conjugated bile acids were hydrolyzed and also, the amounts of oxo bile acids used were recovered without any significant decomposition.
Key words: alkaline hydrolyzing conditions; capillary gas chromatography; conjugated bile acids; 1 N NaOH solution; oxo bile acids
