RETURNVerwendung der Immunhistologie in der Intraoperativen Schnellschnitt-Diagnose: Protokoll der 10-Minutigen Immunhistologie
Hisao Ito, Hironobu, Adachi, Hironobu Andachi, Takayuki Nishi, Hiroyuki Shirai and Christian Wittekind*
Pathologisches Institut der Universität Tottori (Prof. H. Ito), Yonago 683, Japan und *Abteilung für Pathologie in der Chirurgischen und Urologischen Klinik mit Poliklinik, Friedrich-Alexander-Universität Erlnagen-Nürnberg (Prof. Ch. Wittekind), Deutschland
The possibility of rapid immunohistochemical procedure was examined using indirect method in order to apply the immunohistochemistry to intraoperative frozen diagnosis. Reduction of the incubation time was attained without false negative staining by using 4 or 5 times more concentrated primary and secondary antibodies than those used by routine paraffin sections. 0.01 M Imidazol apparently enhanced the DAB-staining. All the procedures could be successfully carried to be useful at the intraoperative frozen diagnosis.
Key words: immunohistochemistry; frozen section; intraoperative diagnosis