RETURNYonago Acta medica 1992;35:55–66
The Effects of Chronic Uremia on the Kinetics of Active and Diffusive Glucose Transport in Rat Small Intestine in vivo
Shin-ichi Yoshioka, Tsutomu Koumi, Tetsuo Mura, Nobuo Yamaga and Shiro Ikawa
Division of Chemistry, Institute of Steroid Research, Faculty of Medicine, Tottori University, Yonago 683, Japan
The effects of chronic renal failure on the small intestine's active and diffusive transport of glucose were investigated in five-sixths nephrectomized uremic rats and in sham-operated normal rats. Four weeks after the operation, an intestinal glucose absorption test using an in vivo perfusion technique was performed. The active transport of glucose was reduced in rats with chronic renal failure. In contrast, the diffusive transport of glucose was increased in uremic rats. Kinetic analysis of the data of the active glucose transport demonstrated that chronic uremia caused a reduction in the maximum absorptive capacity. Enzymatic analysis showed no significant difference between the activity of Na+-K+-ATPase in the intestinal mucosa in the uremic rats and that in the controls. These results suggest that the reductions in active glucose transport in chronic uremia may not be related to changes in the intestinal Na+-K+-ATPase activity.
Key words: chronic uremia; glucose transport; Na+-K+-ATPase; rat; small intestine