RETURNYonago Acta medica 1992;35:167–173
Immunoelectron Microscopic Demonstration of S-100 Protein and Microtubule-Associated Protein 1B in the Novel Astrocytic Inclusion Bodies of a Patient with Brain Malformation
Shinsuke Kato*‡, Asao Hirano*, Fritz Hertz†, Eisaku Ohama‡, Kei Shimoda§ and Masao Minagawa#
*Division of Neuropathology and †Department of Pathology, Montefiore Medical Center, Bronx, NY 10467, United States, ‡Division of Neuropathology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago 683 and §Department of Pathology and #Department of Neuropsychiatry, Saitama Medical School, Saitama 350-04, Japan
This report concerns the immunoelectron microscopic demonstration of expression of S-100 protein (S 100) and microtubule-associated protein 1B (MAP 1B) by the astrocytic inclusion bodies recently identified in an individual with brain malformation. The presence of the two proteins, together with the reported differences in immunohistochemical profiles and absence of filamentous structures, distinguish the novel inclusions from Rosenthal fibers. By immunoelectron microscopy, the products of reaction with the anti-S 100 antibody appeared as heterogeneous granular deposits and with the antibody to MAP 1B they were randomly scattered throughout the astrocytic inclusions. This is the first demonstration of immunoelectron microscopy of this newly discovered astrocytic inclusions.
Key words: astrocytic inclusion; S-100 protein; microtubule-associated protein 1B; immunoelectron microscopy