RETURNYonago Acta medica 1991;34:185-192
Two-color Analysis of T-cell Subsets in Experimental Allergic Neuritis
Hiroshi Takai and Shigeru Araga
Division of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago 683, Japan
We examined the two-color analyses of T-cell subsets in the peripheral blood, spleen and lymph nodes during the course of experimental allergic neuritis (EAN) in Lewis rats 10, 14, 21, 28 and 35 days after immunization by flow cytometry. In the lymph nodes, the percentages of OX-3 and W3/25 double-positive cells and OX-3 and OX-8 double-positive cells in EAN rats (myelin and adjuvant-treated rats) were significantly higher than in the controls (adjuvant-treated rats) 10 days after immunization. In the lymph nodes, the percentages of OX-22 and W3/25 double-positive cells and OX-22 and OX-8 double-positive cells in EAN rats were also significantly higher than in the controls 35 days after immunization. In the spleen and the peripheral blood, there were no significant differences between the EAN rats and the control rats. These results suggest that the upregulation of these T-cells may play an important role in the pathogenesis of EAN.
Key words: experimental allergic neuritis; two-color analysis; T-cell subsets