RETURNYonago Acta medica 1991;34:155-162
Effect of Partially Deacetylated Chitin Oligomers on Two Macrophage Cell Lines: Comparison with Lipopolysaccharide and Phorbol Myristate Acetate
Takahiko Tanigawa, Yoshinori Tanaka, Hitoshi Sashiwa*, Hiroyuki Saimoto* and Yoshihiro Shigemasa*
Department of Bacteriology, Faculty of Medicine, Tottori University, Yonago 683 and *Department of Materials Science, Faculty of Engineering, Tottori University, Tottori 680, Japan
On 2 macrophage cell lines, A640-BB-2 (clone A2) and J774.16 (clone 16), the effects of partially deacetylated chitin oligomers were compared with those of lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA). The cytotoxicity of both cell clones against L929 cells was strongly activated by 100 µg/mL LPS, but not by 400 µg/mL deacetylated chitin oligomer, even at higher concentrations. Nitroblue tetrazolium reduction by clone 16 cells was strongly enhanced with 100 ng/mL PMA, but the reaction by clone A2 cells was weak. Nitroblue tetrazolium reduction by both cell clones was not elicited with 100 µg/mL LPS or 400 µg/mL deacetylated chitin oligomers by both cell clones. Although continuously elicited clone 16 cells for 20 h did respond to the stimulation with 100 ng/mL PMA or 100 µg/mL LPS, 400 µg/mL 66%-deacetylated chitin oligomer did not show a similar activity. These results indicate that LPS and PMA stimulated both cell clones but that deacetylated chitin oligomers do not have any activating effect at a concentration of 400 µg/mL.
Key words: cytotoxicity; lipopolysaccharide; macrophage cell line; nitroblue tetrazolium dye reduction; partially deacetylated chitin oligomer; phorbol 12; myristate 13-acetate